Method for staining both acid-fast and chromophobic tubercle bacilli with carbolfuschsin

Method for staining both acid-fast and chromophobic tubercle bacilli with carbolfuschsin.

Safer staining method for acid fast bacilli.

To develop a method for staining acid fast bacilli which excluded highly toxic phenol from the staining solution. A lipophilic agent, a liquid organic detergent, LOC High Studs, distributed by Amway, was substituted. The acid fast bacilli stained red; nuclei, cytoplasm, and cytoplasmic elements stained blue on a clear background. These results compare very favourably with acid fast bacilli stai...

Evaluation of a dual-staining method for acid-fast bacilli.

A dual-staining procedure for acid-fast bacilli was found to have poor correlation with the Ziehl-Neelsen and auramine-rhodamine staining techniques.

Pyruvic acid egg medium for tubercle bacilli.

Sputum culture for tubercle bacilli on a solid egg medium containing glycerol was compared with culture on a similar medium containing pyruvic acid and no glycerol. Tubercle bacilli from the sputum of five out of 99 patients grew on pyruvic acid medium but not on glycerol medium. The addition of pyruvic acid is therefore essential when an egg medium is used for the cultural diagnosis of tubercu...

Evaluation of a Cold Staining Method for Detecting Acid Fast Bacilli in the Sputum

Tuberculosis is a disease of great importance in developing countries, such as India, where it has caused considerable morbidity and mortality. Sputum smear microscopy is the cornerstone in diagnosis of pulmonary tuberculosis, which is usually done using Ziehl–Neelsen (ZN) method (Vasanthakumari et al., 1986; Gupta et al., 2009; Chandrasekaran et al., 1991). The use of ZN Method in remote areas...